Epithelial‑mesenchymal transition in chronic rhinosinusitis (CRS) and the prognostic value of α‑SMA in postoperative outcomes of patients with CRS
- Authors:
- Published online on: July 3, 2019 https://doi.org/10.3892/mmr.2019.10461
- Pages: 2441-2449
Abstract
Introduction
Chronic rhinosinusitis (CRS) is a chronic inflammatory disease involving sinonasal mucosa (1). CRS affects approximately 8% of the Chinese population, and results in serious health consequences (2). Although endoscopic sinus surgery (ESS) can improve the prognosis of patients with CRS in the case of unsuccessful medical therapy, a refractory course presents in a minority of cases. Therefore, the pathogenesis of CRS requires further investigation.
Epithelial-mesenchymal transition (EMT) is a biological process, in which epithelial cells acquire mesenchymal properties and lose their epithelial phenotype (3). The features of EMT include decreased expression of epithelial makers, such as E-cadherin and cytokeratin, and increased expression of mesenchymal markers, namely α-smooth muscle actin (α-SMA), vimentin and fibronectin. EMT plays key roles in crucial cell functions including organogenesis, tumor formation and fibrosis (4,5). Recently, EMT was reported to be involved in a number of airway diseases (6–9). The pathological basis of the symptoms in CRS includes tissue remodeling, which is characteristic of the abnormal deposition of the extracellular matrix (ECM). Currently, a limited number of studies have been conducted that have examined the association between EMT and CRS (10–16). However, these results were controversial and further investigation on this topic is necessary. The evidence from primary nasal epithelial cells (NECs) in patients with CRS remains limited (14–16), whereas the association between EMT markers and clinical features in CRS has been scarcely explored (16). Therefore, the involvement of EMT in CRS is essential for improving the prognosis of these patients.
The present study aimed to examine the expression of EMT markers in sinonasal specimens from CRS and control patients. CRS was divided into two types, namely chronic rhinosinusitis without nasal polyps (CRSsNP) and chronic rhinosinusitis with nasal polyps (CRSwNP) (17). In addition, EMT features were evaluated in primary NECs following transforming growth factor (TGF)-β1 stimulation. The associations between the expression levels of α-SMA and the clinical characteristics of CRS were also determined.
Materials and methods
Subjects
In the present study, 39 patients were recruited, including 23 females and 16 males, aged 11–72 years old (45.85±14.88). All patients underwent endoscopic sinus surgery (ESS) from September 2009 to February 2011 at the Department of Otolaryngology of the Affiliated Eye Ear Nose and Throat Hospital (AEENTH) of Fudan University. Patients with immunodeficiency diseases (human immunodeficiency virus, diabetes, renal disease) were excluded. CRS subjects were diagnosed according to the EPOS-2007 criteria (17). The patients undergoing ESS for benign diseases other than CRS were employed as the control subjects.
Clinical information was recorded prior to surgery, including age, sex, disease duration, sinus surgical history, smoking history, co-existence of allergic rhinitis, asthma, aspirin sensitivity and gastroesophageal reflux disease (GERD). The Lund-Mackay CT score was estimated preoperatively. The visual analogue scale (VAS), the rhinosinusitis outcome measure-31 (RSOM-31) and the Lund-Kennedy endoscopy scores were assessed pre- and post-operatively as previously described (18–20).
The samples were obtained from the ethmoid sinus with edema during surgery or during discharge. These samples were representative of CRS, whereas the samples from the nasal cavity were used as controls. Each sample was divided into 4 specimens for the detection of mRNA and protein expression, for hematoxylin and eosin (H&E) staining and for immunofluorescence staining, respectively.
Reverse transcription-quantitative PCR (qPCR)
Total RNA was extracted from tissues with TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and subsequently reverse transcribed into cDNA with a SuperScript™ first strand synthesis kit (Invitrogen; Thermo Fisher Scientific, Inc.). RT-qPCR was performed with a thermal cycler (Applied Biosystems; Thermo Fisher Scientific, Inc.). Amplification conditions for the RT reaction were 42°C for 50 min and 70°C for 15 min. qPCR was then performed using an SYBR ExScript kit (Takara Biotechnology Co., Ltd.) and conducted on a Mx3000 Real-Time PCR system (Stratagene, La Jolla, CA). Conditions of the PCR amplification were: 94°C (5 min), then 30 cycles of 94°C for 30 sec, 56°C for 30 sec and 72°C for 60 sec, with a final extension at 72°C for 10 min. Each sample was measured in triplicate. The mRNA levels were determined using the 2−ΔΔCq method (21). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was selected as the control gene. The primer sequences are presented in Table SI.
Western blot analysis
The tissues were incubated with lysis buffer (Beyotime Institute of Biotechnology) on ice. The protein concentration was quantified using a BCA protein concentration assay kit (Beyotime Institute of Biotechnology). The proteins were separated by SDS-PAGE and were loaded on the 10% gels in equal amounts (40 µg). Following electrophoresis, they were transferred to PVDF membranes. The membranes were blocked using 5% BSA (Beyotime Institute of Biotechnology) for 1 h at room temperature, and immunoblotted with mouse monoclonal antibodies against α-SMA (1:1,000; sc-130617; Santa Cruz Biotechnology, Inc.), TGF-β1 (1:1,000; ab64715; Abcam), E-cadherin (1:1,000; ab1416; Abcam), fibronectin (1:1,000; ab26245; Abcam) and GAPDH (1:1,000; cat. no. 97166; Cell Signaling Technology) overnight at 4°C. The following morning, the membranes were incubated with anti-mouse IgG conjugated to horseradish peroxidase (1:2,000; cat. no. 7076; Cell Signaling Technology). The blots were visualized by Amersham ECL™ Select (GE Healthcare Life Sciences) with X-OMAT BT film (Carestream Health). Quantity One 2.0 (Bio-Rad Laboratories, Inc.) was used for the quantification of protein expression. The protein level of control group was set as 1; all data were normalized to the control group.
Immunofluorescence and H&E staining
The tissue samples were fixed with 4% paraformaldehyde for 24 h at room temperature and embedded in paraffin. Embedded tissues were cut into 5-µm sections and deparaffinized with xylene and hydrated with a series of ethanol solutions in a decreasing concentration gradient. The sections were permeabilized with 0.1% Triton X-100, blocked with 1% BSA for 1 h at room temperature, and subsequently incubated with primary antibodies overnight at 4°C, including mouse monoclonal antibodies against TGF-β1 (1:500; ab64715; Abcam), matrix metalloproteinase (MMP)-9 (1:500; ab58803; Abcam) and fibronectin (1:100; ab26245; Abcam). The sections were probed with goat anti-mouse cyanine3-labeled secondary antibody (1:400; M30010, Invitrogen; Thermo Fisher Scientific, Inc.) for 2 h at room temperature, and subsequently mounted with DAPI for 20 min at room temperature. Fluorescence was measured over 10 fields per sample at magnification, ×400 (×40 objective lens) by fluorescence microscopy (Leica Microsystems GmbH).
For double-labeling immunofluorescence, the primary antibodies used were the following: mouse monoclonal antibody against α-SMA (1:200; sc-130617; Santa Cruz Biotechnology, Inc.) and rabbit monoclonal antibody against pan-cytokeratin (1:200; ab234297; Abcam). FITC-conjugated rabbit anti-mouse (1:100; F9137; Sigma-Aldrich; Merck KGaA) and cyanine3-conjugated goat anti-rabbit secondary antibodies (1:200; A10520; Thermo Fisher Scientific, Inc.) were applied respectively.
Furthermore, the pathological classification of Hellquist was conducted for CRSwNP based on H&E staining, as described previously (22).
Isolation of primary NECs
Each tissue was cut into small pieces and incubated with collagenase for 12–24 h at 4°C, as soon as the sinonasal mucosa was removed from the patients with CRS. The suspension was vortexed and filtered (50 µm) to remove the clumps. Magnetic cell sorting method was used to obtain NECs. The cells were resuspended in degassed buffer [phosphate-buffered saline (PBS) pH 7.2, 0.5% FCS, 2 ml EDTA] at a density of 107 cells/80 µl and incubated with microbeads conjugated to monoclonal mouse anti-human CD326 antibodies (Miltenyi Biotec, Inc.) at a bead-to-total cell ratio of 20 µl:107 cells for 20 min at 4°C. The supernatant was aspirated by collecting the beads using an MS Column and a MACS Separator (Miltenyi Biotec, Inc.). The cells were resuspended in BEBM media and seeded in a culture flask (Fig. S1A).
The purity of NECs was verified via two methods. First, immunofluorescence was performed on cells, which were fixed and stained with pan-cytokeratin as described above. The proportions of the NECs (cytokeratin-positive cells) in ten fields were recorded under a fluorescence microscope. Second, flow cytometry was performed. Cells were digested with 0.25% trypsin at 37°C for 5 min and collected via centrifugation at 1,000 × g for 5 min at 4°C. Following two washes with ice-cold PBS, the cells were re-suspended in 50 µl of PBS, blocked with anti-human IgG (1:1,000; cat. no. ab195574; Abcam) at room temperature for 15 min, and subsequently incubated with mouse phycoerythrin-conjugated anti-pan-cytokeratin (1:1,000; cat. no. SAB4700668; Sigma-Aldrich; Merck KGaA) for 30–45 min at 4°C in the dark. The NECs were identified using an EPICS XL flow cytometer (Beckman Coulter, Inc.) and analyzed by Cell Quest software version FCS2.0 (BD Biosciences). Cytokeratin-positive cells were considered NECs. The purity of the NECs reached 89.3% by immunofluorescence or 92.1% by flow cytometry, respectively (Fig. S1B and C).
EMT induction and assessment
For EMT induction, the cells were incubated with TGF-β1 (1, 5 and 10 ng/ml) for 1, 2 and 3 days, respectively. The morphology of the cells was observed using a phase contrast microscope. Double-labeling immunofluorescence of α-SMA and cytokeratin was applied. The expression levels of E-cadherin and α-SMA were determined by western blot analysis, as described above.
Statistical analysis
Statistical analyses were carried out using SPSS 14.0 (SPSS, Inc.). The data are presented as mean ± standard deviations (SD). The differences were analyzed by one-way ANOVA followed by Bonferroni post hoc test among multiple groups. The Kruskal-Wallis test was employed for data with skewed distribution. The correlations between α-SMA levels and clinical parameters of CRS were calculated by the Pearson correlation method. P<0.05 was used to indicate significant differences.
Results
Basic characteristics
A total of 26 patients with CRS and 13 control subjects were enrolled in the present study. Patients with CRS included 13 CRSwNP and 13 CRSsNP cases. The controls involved 3 cases with paranasal sinus cyst, 3 cases with cerebrospinal fluid rhinorrhea, 2 cases with nasal septum deviation, 2 cases with traumatic optic neuropathy, 1 case with pituitary adenoma, 1 case with thyroid eye disease and 1 case with frontal sinus osteoma. The basic characteristics are documented in Table SII.
Expression of EMT markers in tissues of patients with CRS and control subjects
The mRNA levels of E-cadherin in CRS group were significantly increased compared with in the control group, whereas, no significant differences were observed between the two groups in the mRNA levels of other markers including α-SMA, TGF-β1, MMP-9, fibronectin and vimentin (Table SIII). The mRNA levels of E-cadherin, α-SMA and TGF-β1 in CRSsNP were significantly higher than those noted in the control subjects. CRSwNP exhibited decreased mRNA levels of α-SMA and vimentin compared with the expression levels of these markers in the control subjects. CRSsNP tissues had elevated expression of E-cadherin, α-SMA, TGF-β1, fibronectin and vimentin when compared with CRSwNP. MMP-9 mRNA levels exhibited no difference between these two groups (Fig. 1A-C).
The protein levels of E-cadherin in CRS were significantly higher than those noted in the control subjects. The protein levels of α-SMA, TGF-β1 and E-cadherin in CRSsNP were significantly increased compared with the control group. The protein levels of α-SMA, TGF-β1 and fibronectin in CRSsNP were significantly upregulated compared with CRSwNP (Fig. 1D-H). The protein levels of the EMT markers are presented in Table SIV.
The protein levels of α-SMA, TGF-β1, fibronectin and MMP-9 were increased in CRSsNP, as demonstrated by immunofluorescence assays (Fig. 2). In addition, the cells that co-expressed α-SMA and cytokeratin were detected in the mucosal epithelium in both CRSsNP and CRSwNP using double-labeling immunofluorescence assays (Fig. 2A).
Characteristics of EMT in primary NECs following TGF-β1 treatment
Following incubation with TGF-β1, the NECs acquired a mesenchymal phenotype as demonstrated by phase contrast microscopy. After 24 h of induction by TGF-β1, the junctions among epithelial cells were interrupted, and the cells became dispersed and flat. After 3 days, the cytoplasm began to show polarity, extending in two opposite directions like tentacles. After 7 days, over half of the cells presented typical long spindle fibroblasts (Fig. 3A). In addition, NECs that co-expressed α-SMA and cytokeratin were detected following TGF-β1 treatment by double-labeling immunofluorescence staining (Fig. 3A and B). In addition, the protein levels of α-SMA were increased, whereas the levels of E-cadherin were decreased following TGF-β1 treatment (Fig. 3C).
Correlation between α-SMA protein expression and clinical features of CRS
A negative correlation was evident between α-SMA protein expression and smoking. No association existed between α-SMA levels and the remaining clinical characteristics (Table I).
With regard to preoperative parameters, no correlation was noted between α-SMA levels and the symptoms noted in RSOM-31 (Table SV). The α-SMA levels were inversely correlated with the Lund-Kennedy score, while they did not correlate with specific endoscopic signs (Table II). α-SMA levels were negatively correlated with CT scores of anterior ethmoid sinus, while no correlation was noted with the other paranasal sinuses, indicating that CRS simply involving anterior ethmoid were less likely to undergo EMT (Table SVI).
Correlation between α-SMA protein expression and surgical outcomes of CRS
The levels of α-SMA were negatively correlated with RSOM-31 symptoms including hyposmia, hearing loss, fatigue, and reduced productivity (Table III). A trend towards negative correlation was observed between α-SMA levels and VAS score, although no significance was found. Negative correlations were present between α-SMA levels and endoscopic score, and between α-SMA levels and endoscopic signs, including polyps, discharge and crusting (Table IV).
Table III.Correlation analysis between α-SMA and postoperative symptom scores of the patients with CRS. |
Table IV.Correlation analysis between α-SMA and postoperative endoscopic scores of the patients with CRS. |
Discussion
Chronic rhinosinusitis (CRS) is a prevalent disease in otolaryngology. It affects the quality of life (QOL) of patients and imposes a heavy burden on socioeconomic resources (23). The abnormal deposition of extracellular matrix (ECM) in the mucosa is one of the pathological causes of CRS. Epithelial-mesenchymal transition (EMT) plays crucial roles in tissue remodeling in several pathophysiological processes (24). Recently, the induction of EMT was reported in CRS by several studies (10–16). Nevertheless, inconsistent results were provided. A limited number of studies have indicated that EMT is involved in patients with CRS with nasal polyps (CRSwNP) and not in patients with CRS without polyps (CRSsNP) (10–12), while Hupin et al demonstrated increased vimentin expression levels in both groups (16). The majority of these studies explored the association of CRSwNP with the aforementioned markers, while a limited number of studies focused on CRSsNP. Therefore, additional evidence is required to unravel the involvement of EMT markers in CRSsNP. In the present study, the cells were stained using double-labeling with α-SMA and cytokeratin, indicating the induction of EMT in CRS. To the best of our knowledge, this is the first study that utilized double-label staining to assess EMT induction in CRS. Double-label staining is an intuitive evidence for EMT that illustrates whether cells are undergoing this process. Several double-label stained cells were observed in both CRSsNP and CRSwNP, although the fluorescence noted was weak in CRSwNP. EMT can elicit loss of epithelial markers and gain of mesenchymal markers. Increased expression of epithelial (E-cadherin) and mesenchymal markers (α-SMA, vimentin, fibronectin, MMP-9) was noted in both CRSsNP and CRSwNP. This is inconsistent with the findings of Konnecke et al suggesting that the induction of EMT was partially present in CRS (15). Partial EMT was proposed in oncology to describe an intermediate stage of EMT, which generates cancer cells with a higher degree of malignancy than those with a complete EMT phenotype (25,26). However, the underlying mechanisms require further investigation.
In contrast to previous studies, the present report indicated different EMT features in CRSsNP than those noted in CRSwNP, which may be attributed to histological distinctions. Edematous and fibrous types are common histological patterns of nasal polyps (NPs) (22). Edematous NPs exhibit a thin layer of epithelium and a lower number of fibroblasts in ECM compared with those noted in fibrous NPs. In the present study, edematous and fibrous types comprised 84.6 and 15.4% of the NPs, respectively (Fig. S2). Since pathological classification was not conducted in the previous studies, the corresponding comparisons with the present study could not be performed. This suggests that further investigations are required for a more accurate conclusion (10–12).
Although EMT was observed in the nasal epithelial cells (NECs), several studies conducted previously used cell lines as models instead of primary NECs (11–13). Moreover, certain studies have investigated NECs derived from NPs (10), and reports on primary NECs from CRSsNP remain limited (14–16). The incidence of CRSsNP is higher than that of CRSwNP and the two types of CRS may have distinct pathogenic mechanisms. Therefore, it is of great significance to understand the pathogenesis of CRSsNP. We investigated EMT-related features including morphology and expression of the relevant markers in primary NECs. Following TGF-β1 exposure, NECs acquired a mesenchymal shape. In addition, NECs co-expressing α-SMA and cytokeratin were detected and TGF-β1 was able to induce α-SMA expression and inhibit E-cadherin expression in NECs. The present study validated that NECs could respond to TGF-β1 in a time dependent manner, which is consistent with previous research (15). Taken collectively, the data demonstrated that primary NECs from CRS were able to undergo EMT in response to TGF-β1 treatment.
The TGF-β1 pathway plays a key role in EMT of many pathological conditions (27). TGF-β1 regulates EMT through canonical or non-canonical pathways. The canonical pathway is the SMAD signaling pathway. When TGF-β1 binds to TGF-β receptor II (TGFβR II), the cascade signaling is initiated. TGFβR II recruits and phosphorylates TGFβR I. TGFβR I then phosphorylates R-Smad which is a complex formed with Smad2 and Smad3. Phosphorylated R-Smad forms Co-Smad by binding to Smad4, which then shuttles into the nucleus to regulate transcription via binding to target genes (28). The non-canonical pathways are triggered when the TβRI/II complex activates certain signals such as MAPK, p38 or JNK pathways (27). Currently, it has not been determined whether canonical or non-canonical TGF-β1 pathways are implicated in EMT of CRS, which warrants further investigation in the future.
The association between EMT markers and clinical parameters in CRS has been scarcely investigated. Hupin et al reported that increased expression of vimentin was associated with CT score (16). Therefore, the investigation of the association of EMT markers with the clinical features of CRS is helpful for the treatment of such patients. In the present study, partial EMT was noted in the sinus mucosa of patients with CRS, which exhibited a different expression pattern of epithelial markers compared with that noted in the typical EMT process. In sinonasal mucosa, fibroblast markers, such as fibronectin and vimentin are expressed not only in fibroblasts, but also in endothelial cells, while the α-SMA protein is specifically expressed in myofibroblasts (29). Therefore, we assessed the correlation between α-SMA levels and clinical characteristics of patients with CRS. In the present study, no association was noted between α-SMA levels and total CT score. It is interesting to note that a negative correlation was found between α-SMA levels and CT score of the anterior ethmoids, suggesting that CRS involving anterior ethmoid was less likely to undergo EMT. This may be related to the anatomical differences of the tissues, since natural drainage of the anterior ethmoid is usually better than that of the other paranasal sinuses. Furthermore, this is the first study to evaluate the prognostic value of the EMT marker α-SMA in the surgical outcomes of CRS subjects. Although α-SMA was not associated with preoperative symptoms, it was negatively correlated with postoperative symptoms including hyposmia, hearing loss, fatigue and reduced productivity. These were systemic symptoms rather than nasal symptoms, suggesting that the use of α-SMA as a marker was more relevant to the overall QOL of CRS following surgery. With regard to the endoscopic parameters, α-SMA levels were negatively correlated with preoperative and postoperative scores, and with postoperative signs including polyps, discharge and crusting. These unexpected results provided the first preliminary evidence showing that EMT could predict the surgical outcome of CRS subjects. This hypothesis requires verification using larger sample sizes. According to our clinical experience, the prognosis of CRSsNP is better than that of CRSwNP as a whole, which might be an explanation for the degree of EMT noted in CRSsNP compared with that of CRSwNP.
In conclusion, it was demonstrated that partial EMT occurred in CRS in vivo and that primary NECs responded to TGF-β1 induction in vitro, indicating that EMT is involved in the pathogenesis of CRS. In addition, we demonstrated that α-SMA could be a predictor for improved postoperative endoscopic and symptomatic outcomes in CRS. These findings require further validation using a large cohort study.
Supplementary Material
Supporting Data
Acknowledgements
Not applicable.
Funding
The present study was supported by the National Natural Science Foundation of China (grant nos. 8187040939 and 81600783).
Availability of data and materials
All the data generated and analyzed in the present study are available from the corresponding author upon reasonable request.
Authors' contributions
HL, XCS and DHW conceived and designed the experiments. HL, HW and QL performed the experiments. LH, HPY and QL analyzed the data and conducted the statistical analysis. HL and HW wrote the manuscript. All the authors read and approved the final version of the manuscript and agree to be accounTable for all aspects of the research in ensuring that the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Ethics approval and consent to participate
The present study was approved by the Ethics Committee of AEENTH and written informed consent was obtained from every patient.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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